If you are doing research in biology, you will have to know how to use the Genome browsers:
UCSC genome browser, Ensemble, and the NCBI genome browser.
See a introduction here:
https://www.youtube.com/watch?v=vtJ6FvwCVTM&list=PLdmZ_rDf9sGScxV3bCR_QpDYaUpBY_CEA
I use UCSC genome browser a lot, mostly because of the rich annotation tracks. Sometimes, I go to Ensemble, it has a better view of sequences of exons and introns etc. I barely use the NCBI genome browser.
For Next Generation Sequencing analysis, you will be using some local genome browsers
IGV from Broad Institute http://www.broadinstitute.org/igv/
SeqMonk ,http://www.bioinformatics.babraham.ac.uk/projects/seqmonk/ it is excellent for some quantitation analysis of ChIP-seq, RNA-seq data.
MochiView, http://johnsonlab.ucsf.edu/mochi.html
it is really good for motif analysis besides the genome browsing function.
IGB http://bioviz.org/igb/, I have not used it personally
other browsers like Gbrowser
http://gmod.org/wiki/GBrowse
from the GMOD project is also an alternative.
http://gmod.org/wiki/Main_Page
Artemis from sanger Institute:
http://www.sanger.ac.uk/resources/software/artemis/
see a wiki link here: http://en.wikipedia.org/wiki/Genome_browser
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