In the protocol it requires fastq files listed as: *001_R1.fastq.gz
001 is the replicate number, it can be 002 or 003 or any number of replicates you have. ( for RNA-seq, sequence as many as biological samples as possible !)
R1 is the pair-end reads 1, it can be R2
What he has is something like:
1_egg_r1_01_sub.fastq.gz
1 is the stage of the egg. He sequenced 4 eggs, so he has 1_egg, 2_egg., 3_egg and 4_egg
r1 is the pair-end reads 1
01 is the first replicates. He has two replicates for each egg.
Basically, he wants to rename these files to the khmer convention.
This problem gets down to writing a regular expression.
To recapture the problem, I made some dummy files:
mkdir foo && cd foo
I have a txt file contains the names of the file:
foo$ cat files.txt
1egg_r1_01_sub.fastq.gz
1egg_r2_01_sub.fastq.gz
1egg_r1_02_sub.fastq.gz
1egg_r2_02_sub.fastq.gz
2egg_r1_01_sub.fastq.gz
2egg_r2_01_sub.fastq.gz
2egg_r1_02_sub.fastq.gz
2egg_r2_02_sub.fastq.gz
3egg_r1_01_sub.fastq.gz
3egg_r2_01_sub.fastq.gz
3egg_r1_02_sub.fastq.gz
3egg_r2_02_sub.fastq.gz
4egg_r1_01_sub.fastq.gz
4egg_r2_01_sub.fastq.gz
4egg_r1_02_sub.fastq.gz
4egg_r2_02_sub.fastq.gz
Now I want to make dummy files with the names in this file.
one can make the dummy files in a fly also.
=====update on 08/26/14======
one can use the {} expansion to create the dummy files
tommy@tommy-ThinkPad-T420[foo] touch {1,2,3,4}_r{1,2}_0{1,2}_sub.fastq.gz
tommy@tommy-ThinkPad-T420[foo] ls [ 3:45PM]
1_r1_01_sub.fastq.gz 2_r2_01_sub.fastq.gz 4_r1_01_sub.fastq.gz
1_r1_02_sub.fastq.gz 2_r2_02_sub.fastq.gz 4_r1_02_sub.fastq.gz
1_r2_01_sub.fastq.gz 3_r1_01_sub.fastq.gz 4_r2_01_sub.fastq.gz
1_r2_02_sub.fastq.gz 3_r1_02_sub.fastq.gz 4_r2_02_sub.fastq.gz
2_r1_01_sub.fastq.gz 3_r2_01_sub.fastq.gz
2_r1_02_sub.fastq.gz 3_r2_02_sub.fastq.gz
========================
=====update on 08/26/14======
one can use the {} expansion to create the dummy files
tommy@tommy-ThinkPad-T420[foo] touch {1,2,3,4}_r{1,2}_0{1,2}_sub.fastq.gz
tommy@tommy-ThinkPad-T420[foo] ls [ 3:45PM]
1_r1_01_sub.fastq.gz 2_r2_01_sub.fastq.gz 4_r1_01_sub.fastq.gz
1_r1_02_sub.fastq.gz 2_r2_02_sub.fastq.gz 4_r1_02_sub.fastq.gz
1_r2_01_sub.fastq.gz 3_r1_01_sub.fastq.gz 4_r2_01_sub.fastq.gz
1_r2_02_sub.fastq.gz 3_r1_02_sub.fastq.gz 4_r2_02_sub.fastq.gz
2_r1_01_sub.fastq.gz 3_r2_01_sub.fastq.gz
2_r1_02_sub.fastq.gz 3_r2_02_sub.fastq.gz
========================
The difference of make_dummy_file.sh and make_dummy_file_1.sh is that I specified shebang line in the make_dummy_file.sh script to tell the bash that it is a bash script, to invoke it: ./make_dummy_file.sh files.txt
In contrast, to invoke the other two which I did not specify the shebang: bash make_dummy_file_1.sh bash make_dummy_file_2.sh
Rename the files with regular expression by either using sed or rename command
In contrast, to invoke the other two which I did not specify the shebang: bash make_dummy_file_1.sh bash make_dummy_file_2.sh
Rename the files with regular expression by either using sed or rename command
the sed command need to escape the () which are used to capture the back reference
before:
tommy@tommy-ThinkPad-T420:~/foo$ ls 1_egg_r1_01_sub.fastq.gz 2_egg_r1_01_sub.fastq.gz 3_egg_r1_01_sub.fastq.gz 4_egg_r1_01_sub.fastq.gz copy make_dummy_file_1.sh 1_egg_r1_02_sub.fastq.gz 2_egg_r1_02_sub.fastq.gz 3_egg_r1_02_sub.fastq.gz 4_egg_r1_02_sub.fastq.gz dummy make_dummy_file_2.sh 1_egg_r2_01_sub.fastq.gz 2_egg_r2_01_sub.fastq.gz 3_egg_r2_01_sub.fastq.gz 4_egg_r2_01_sub.fastq.gz files.txt rename.sh 1_egg_r2_02_sub.fastq.gz 2_egg_r2_02_sub.fastq.gz 3_egg_r2_02_sub.fastq.gz 4_egg_r2_02_sub.fastq.gz make_dummy_file.sh rename_one_liner.sh
after:
tommy@tommy-ThinkPad-T420:~/foo$ ls 1egg_R1_001.fastq.gz 2egg_R1_001.fastq.gz 3egg_R1_001.fastq.gz 4egg_R1_001.fastq.gz copy make_dummy_file_1.sh 1egg_R1_002.fastq.gz 2egg_R1_002.fastq.gz 3egg_R1_002.fastq.gz 4egg_R1_002.fastq.gz dummy make_dummy_file_2.sh 1egg_R2_001.fastq.gz 2egg_R2_001.fastq.gz 3egg_R2_001.fastq.gz 4egg_R2_001.fastq.gz files.txt rename.sh 1egg_R2_002.fastq.gz 2egg_R2_002.fastq.gz 3egg_R2_002.fastq.gz 4egg_R2_002.fastq.gz make_dummy_file.sh rename_one_liner.sh
References: http://stackoverflow.com/questions/399078/what-special-characters-must-be-escaped-in-regular-expressions
http://stackoverflow.com/questions/10929453/bash-scripting-read-file-line-by-line
https://www.cs.tut.fi/~jkorpela/perl/regexp.html
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