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Tuesday, August 27, 2013

Two nature protocols for RNA-seq analysis

One is from Simon Anders, his group wrote the DESeq

http://www.nature.com/nprot/journal/v8/n9/full/nprot.2013.099.html

Count-based differential expression analysis of RNA sequencing data using R and Bioconductor



The other one 


Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks


I will go through a complete RNA-seq analysis following the guidelines.
There are other packages make RNA-seq analysis easier:

Lots of things are waiting for me to try out!





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