15 tools/papers for multi-sample multi-group single-cell RNAseq differential expression analysis

 1/  [An Empirical Bayes Method for Differential Expression Analysis of Single Cells with Deep Generative Models](https://www.biorxiv.org/content/10.1101/2022.05.27.493625v1) scVI-DE

2/  [muscat](http://www.bioconductor.org/packages/release/bioc/html/muscat.html)

3/  [Confronting false discoveries in single-cell differential expression](https://www.nature.com/articles/s41467-021-25960-2) "These observations suggest that, in practice, pseudobulk approaches provide an excellent trade-off between speed and accuracy for single-cell DE analysis." One needs to considder biolgoical replicates, pseduobulk works well.

4/  [Modelling group heteroscedasticity in single-cellRNA-seq pseudo-bulk data](https://www.biorxiv.org/content/10.1101/2022.09.12.507511v1)

5/  [BSDE: barycenter single-cell differential expression for case–control studies](https://academic.oup.com/bioinformatics/article/38/10/2765/6554192?login=false)

 6/ [distinct](http://www.bioconductor.org/packages/release/bioc/html/distinct.html) Both are from Mark Robinson group.

7/ [nebula](https://github.com/lhe17/nebula) https://www.biorxiv.org/content/biorxiv/early/2020/09/25/2020.09.24.311662.full.pdf

8/  [Fast identification of differential distributions in single-cell RNA-sequencing data with waddR](https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioinformatics/btab226/6207964) https://github.com/goncalves-lab/waddR

9/ [CoCoA-diff: counterfactual inference for single-cell gene expression analysis](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-021-02438-4)

10/ [Bias, robustness and scalability in single-cell differential expression analysis](https://www.nature.com/articles/nmeth.4612) From Mark Robinson group.

11/ [Comparative analysis of differential gene expression analysis tools for single-cell RNA sequencing data](https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2599-6) "We observed that current methods designed for scRNAseq data do not tend to show better performance compared to methods designed for bulk RNAseq data."

12/  [Tree-based Correlation Screen and Visualization for Exploring Phenotype-Cell Type Association in Multiple Sample Single-Cell RNA-Sequencing Experiments](https://www.biorxiv.org/content/10.1101/2021.10.27.466024v1) TreeCorTreat is an open source R package that tackles this problem by using a tree-based correlation screen to analyze and visualize the association between phenotype and transcriptomic features and cell types at multiple cell type resolution levels.

13/ [Quantifying the effect of experimental perturbations in single-cell RNA-sequencing data using graph signal processing](https://www.biorxiv.org/content/10.1101/532846v3) read this thread https://twitter.com/krishnaswamylab/status/1328876444810960896?s=27

14/  [Causal identification of single-cell experimental perturbation effects with CINEMA-OT](https://www.biorxiv.org/content/10.1101/2022.07.31.502173v1)

github https://github.com/vandijklab/CINEMA-OT

15/ [IDEAS: individual level differential expression analysis for single-cell RNA-seq data](https://genomebiology.biomedcentral.com/articles/10.1186/s13059-022-02605-1)