tag:blogger.com,1999:blog-4376119613549496246.post4490307916974284489..comments2024-03-27T23:36:17.389-07:00Comments on Diving into Genetics and Genomics: RNA-seq analysis samtools sort and htseq-counttommyhttp://www.blogger.com/profile/04023008941349107659noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-4376119613549496246.post-31861911670212662842021-11-01T22:44:49.088-07:002021-11-01T22:44:49.088-07:00All thanks to Mr Anderson for helping with my prof...All thanks to Mr Anderson for helping with my profits and making my fifth withdrawal possible. I'm here to share an amazing life changing opportunity with you. its called Bitcoin / Forex trading options. it is a highly lucrative business which can earn you as much as $2,570 in a week from an initial investment of just $200. I am living proof of this great business opportunity. If anyone is interested in trading on bitcoin or any cryptocurrency and want a successful trade without losing notify Mr Anderson now.Whatsapp: (+447883246472 )<br />Email: tdameritrade077@gmail.comMARGARET MAGOTHEhttps://www.blogger.com/profile/11818424528397171745noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-51386196972713700252019-03-08T05:04:56.367-08:002019-03-08T05:04:56.367-08:00Dear tommy and freinds, hello, I used HTseq count ...Dear tommy and freinds, hello, I used HTseq count for my data. As an imput I just put a sam file in the command line of HTseq and ultimately I got a file output. Then I imported to my R studio to analyses RNA seq by the DESEQ2. But I don not know how i can use the HTseq count columns or variable received by HTseq as input for deseq2. For example the first columns of my HTseq conut output is the name of genes list but I do not know other columns and how they should be used in Rstudio for the DEseq analyzing. Would you please help me out and tell me which columns of HTseq should be considered after introducing them?<br />my eamil is hamid_fiuji@yahoo.com i would appreciate if you could send your reply to my <br />email<br />sincerely yours<br />Hamid Anonymoushttps://www.blogger.com/profile/04002028289550085036noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-6099003125090538662016-03-28T13:11:09.738-07:002016-03-28T13:11:09.738-07:00I just googled and found this http://seqanswers.co...I just googled and found this http://seqanswers.com/forums/showthread.php?t=36022tommyhttps://www.blogger.com/profile/04023008941349107659noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-35489636948537032732016-03-25T22:43:21.014-07:002016-03-25T22:43:21.014-07:00I use htseq-count for a RNAseq, it constantly give...I use htseq-count for a RNAseq, it constantly gives error Error occured when reading beginning of SAM/BAM file.<br /><br /> [Exception type: StopIteration, raised in count.py:84]. Do you know how to solve this problem. ThanksAnonymoushttps://www.blogger.com/profile/00854016385437558889noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-61886494556403968112015-11-11T11:51:42.610-08:002015-11-11T11:51:42.610-08:00go to NCBI GEO and search http://www.ncbi.nlm.nih....go to NCBI GEO and search http://www.ncbi.nlm.nih.gov/geo/tommyhttps://www.blogger.com/profile/04023008941349107659noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-10293861414447360692015-11-05T16:16:36.142-08:002015-11-05T16:16:36.142-08:00Where can I download the SRA files?Where can I download the SRA files?Rayhttps://www.blogger.com/profile/12624359999611834150noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-86493031268242549582015-04-30T15:13:47.387-07:002015-04-30T15:13:47.387-07:00Thank you, I keep learning everyday. I am aware of...Thank you, I keep learning everyday. I am aware of featureCounts and it looks like it runs faster!tommyhttps://www.blogger.com/profile/04023008941349107659noreply@blogger.comtag:blogger.com,1999:blog-4376119613549496246.post-42017682379050968832015-01-06T02:21:11.955-08:002015-01-06T02:21:11.955-08:00Great post Tommy! I have found featureCounts to be...Great post Tommy! I have found featureCounts to be quite useful for read counting. It has many options and is much faster than HT-Seq or Bedtools Multicov. It can even be run from within R.<br />http://www.ncbi.nlm.nih.gov/pubmed/24227677Mark Ziemannhttps://www.blogger.com/profile/00623549232702735102noreply@blogger.com