Thursday, April 20, 2017

SHERLOCK to detect early circulating tumor DNA

Early detection of circulating tumor DNA is quite challenging. Read this comment in Cell:


And here comes SHERLOCK(Specific High Sensitivity Enzymatic Reporter UnLOCKing)!
from Nucleic acid detection with CRISPR-Cas13a/C2c2,  a study leaded by Feng Zhang in MIT.

Abstract:
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting CRISPR effector Cas13a (previously known as C2c2) exhibits a “collateral effect” of promiscuous RNAse activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify cell-free tumor DNA mutations. Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence and long-term storage, and readily reconstituted on paper for field applications.

This may be a game-changer in the field.


Wednesday, April 19, 2017

sshfs on ubuntu and ssh key

Two things I want to keep a note here:

First, if you ever have set up a shh key for connecting to remote server, you need to be aware that password-less shh key only works when your home directory on the server is not 777 (writable by others).

see this stackexchange post .

Second, I was following https://www.cyberciti.biz/faq/how-to-mount-remote-directory-filesystems-with-sshfs-on-linux/ to set up sshfs on my ubuntu machine. I put down a gist below.